There are two kinds of load controlling mode for asphalt mixtures fatigue testing: stress controlling mode and strain controlling mode. However, the latter is more practical. Firstly, five kinds of asphalt mixtures were experimented with the fatigue testing of control strain mode using MTS810. By the experiment, a fatigue function between fatigue life span and the value of repeated strain as the ratio of strain level was set up. In addition, their fatigue properties were analyzed. It is shown that at the same strain level, asphalt mixtrues logarithm fatigue life is normal school, meanwhile, relation of strain level with logarithm fatigue life is linear. In the fatigue testing of control strain mode, when asphalt mixtrues modulus is much lower, its fatigue life is much longer.