According to the theories of economics and traffic engineering, this paper studied the market demand, segmenting market, targeting market, toll pricing method of toll road, analyzed road company behavior, put forward the operation method of road company. The applied results show that the marketing methods can rationalize the operation of road company, and increase the tolling revenue, the regulating methods can control the behavior of road company, and decrease the lost of social welfare. The contents of the paper can direct the operation of toll road company and is also important to the government regulation for toll road.